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The term “chromatogram” means a plot obtained by chromatography. Fig.4 shows an example of a chromatogram. A chromatogram is a two-dimensional plot with the vertical axis representing the concentration in terms of detector signal intensity and the horizontal axis representing the time of analysis. When no compound is eluted from the column, a line parallel to the horizontal axis is plotted. This is called the baseline. The detector responds based on the concentration of the target compound in the elution band. The resulting plot is more bell-shaped than triangular. This shape is called a “peak”.
Retention time (tR) is the time interval between the sample injection point and the peak peak. The time required for non-protected compounds (compounds with no interaction for the stationary phase) to travel from the injector to the detector is called the dead time (t0).
Peak height (h) is the vertical distance between the top of a peak and the baseline, and the light blue peak area (A) is the area enclosed by the peak and the baseline. These results will be used for qualitative and quantitative analysis of the constituents of a sample.
