Principle of HPLC

For Liquid Chromatography High-performance liquid chromatography is an efficient type of column chromatography widely used in pharmaceuticals. It is very useful for determination of assay and related substances in drug substance. In general, HPLC is used to separate the components of a mixed drug substance. In ...

HPLC Method Development

Analytical method development is considered an important process in pharmaceuticals. The availability of different types of columns, operating parameters, mobile phase compositions, diluents and pH values makes it important to develop an analytical method. A good analytical procedure should be simple, ...

10 Tips for HPLC Analysis In Pharmaceuticals

HPLC, also known as High-Performance Liquid Chromatography system, is one of the most crucial analytical methods in pharmaceuticals. The purpose of HPLC is the separation of each compound that make up a mixture. It passes pressurized liquid solvent with the use of pumps which holds the sample mixture ...

Apparatus of HPLC

“Basic overview of HPLC process” and its procedures are now covered In more detail, HPLC consists of various components including a solvent delivery pump, a degassing unit, a sample injector, a column oven, a detector. , and a data processor. HPLC flow diagram showing the role of each component. As ...

HPLC

HPLC is an acronym for High Performance Liquid Chromatography. “Chromatography” is a separation technique, “chromatogram” is the result of chromatography, and “chromatograph” is the instrument used to conduct chromatography. Among the various technologies developed ...
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