Follow
1.0 OBJECTIVE : To set up a guideline for the operation and cleaning of B.O.D. (Biological Oxygen Demand) Incubator (Instrument ID: QC/140).
2.0 SCOPE : This SOP is applicable for operation and cleaning of B.O.D. Incubator (Instrument ID: QC/140).
3.0 RESPONSIBILITY
3.1 Operating personnel is responsible for carrying out the procedure.
3.2 Overall responsibility for training, implementation & follow-up with the QC Manager or his/her nominee.
4.0 ACCOUNTABILITY
4.1 Head of Quality.
5.0 PRECAUTIONS
5.1 Ensure the door is properly closed while running the chamber.
5.2 Do not keep the chamber in high temperature area.
5.3 Always keep the inside of the incubator clean.
5.4 Do not keep the incubator opened for longer period during loading or unloading samples.
6.0 PROCEDURE
6.1 Operation
6.1.1 Turn ‘ON’ the main switch provided on the main control panel.
6.1.2 All of the display should be ON when the main switch is ‘ON’ (Display of the Temperature controller and Inside Chamber Motor ON).
6.1.3 The lower part of the temperature controller display will show the set temperature and upper part will show the actual inside temperature of the incubator.
6.1.4 Set the desired temperature (i.e. 23°C) by pressing increment key (▲) [to increase the temperature] or decrement key (▼) [to decrease the temperature].
6.1.5 Set the safety controller with the same set temperature as that set on the temperature controller. Also set the required alarm band and initial time. The safety controller will cut of the mains contactor in case of overshoot or undershoot of the temperature above the alarm band. In case when the safety controller trips, one has to reset the safety controller to restart the chamber.
6.1.6 When the temperature is reached to the desired level, place culture plates in the incubator for required period of time.
6.1.7 After incubation collect the culture plates.
6.1.8 Chamber light should be switched ‘ON’ only for the observation purpose or to illuminate the internal chamber while loading or unloading. Normally the chamber light should be kept ‘OFF’.
6.1.9 Monitor the displayed temperature of the incubator on routine basis (twice daily) and record the temperature in the respective record sheet (Format: FQC/282-00).
6.1.10 Count the nos. of plates /bottles kept in and taken out from the incubator and record this in the respective log register as mentioned in Annexure-I (Format: FQC/315-03).
6.1.11 Record the used media lot number, in time & out time of the plates/bottles in the log register Annexure-I (Format: FQC/315-03).
6.1.12 In case there is any anomaly found in the incubator (e.g. temperature), immediately call the factory engineer’s or supplier engineers to attend the incubator.
6.2 Cleaning
6.2.1 Clean inside of the chamber after every seven days with clean cloth soaked with 70% IPA.
6.2.2 Clean outer surface and all parts of instrument carefully once a week.
6.2.3 Clean the shelves with detergent and purified water once in three months. Then wipe with clean cloth soaked with 70% IPA.
6.2.4 Clean the PT-100 sensor tip with the help of a smooth polish paper every month.
