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“Basic overview of HPLC process” and its procedures are now covered In more detail, HPLC consists of various components including a solvent delivery pump, a degassing unit, a sample injector, a column oven, a detector. , and a data processor. HPLC flow diagram showing the role of each component.
As in HPLC, the pump delivers the mobile phase at a controlled flow rate (a). Air can easily dissolve in the mobile phase under the standard atmospheric pressure we live in. If air bubbles are present in the mobile phase and enter the delivery pump, problems such as flow rate fluctuations and baseline noise/flow may occur. Degassing units help prevent this problem by removing air bubbles in the mobile phase(b). After removing the dissolved air, the mobile phase is dispensed into the column. The sample injector then introduces a standard solution or sample solution into the mobile phase (c). Fluctuations in temperature can affect the separation of compounds in the column. The column is placed in a column oven to keep the temperature constant (d). Compounds eluting from the column are detected by a detector placed downstream of the column (E). A workstation processes the signal from the detector to obtain a chromatogram to identify and quantify compound (f).
